Journal: bioRxiv

Article Title: Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models

doi: 10.64898/2026.03.16.711716

Figure Lengend Snippet: CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001

Article Snippet: The following primary antibodies were used: chicken anti-GFAP (1:1000 Aves #GFAP), rat anti-CD31 (1:100 BD Pharmingen 55370), rabbit anti-Albumin (1:500 Invitrogen PA5-85166), conjugated Alexa fluor 647-cleaved caspase-3 (1:100 Bioss bs.20363R-bf647) and conjugated Alexa fluor 488-NeuN (1:100 Millipore ABN78A4).

Techniques: Permeability, Control, Enzyme-linked Immunosorbent Assay, Membrane

Journal: Journal of Assisted Reproduction and Genetics

Article Title: Caspase signalling pathways in human spermatogenesis

doi: 10.1007/s10815-013-9938-8

Figure Lengend Snippet: Relative volumes of active caspases-8, −9 and −3 per germ cell stage per seminiferous tubules

Article Snippet: After 1 h of incubation with 5 % of normal horse serum (RT, Vectastain ABC Kit; diluted in 0.1 % Tween20/PBS), primary antibody was incubated overnight at 4 °C: active caspase-3 (Monoclonal Anti-Human/Mouse Cleaved Caspase-3 Antibody, R&D Systems, Minneapolis, USA; 1:150 diluted in 5 % normal horse serum); active caspase-9 (Monoclonal Anti-Caspase-9 Cleavage Site Specific, Sigma; 1:50 diluted in 5 % normal horse serum).

Techniques: